Phosphorylation is one of the most important PTMs in a biological system. This service can be used to study the phosphorylated proteins in your biological samples. Phosphorylated proteins/peptides will be enriched and then subject to downstream proteome identification or quantification through Mass Spectrometry Analysis. Total phosphorylation proteome (through TiO2 enrichment) and specific targeted phosphorylation proteome (through enrichment using anti-Phospho-Tyrosine or anti-Phospho-(Ser/Thr) Phe antibodies) can be analyzed to identify the phosphorylated proteins in your sample, and the difference in phosphorylation levels of proteins in your samples when combining with TMT or iTRAQ labeling strategy. Your samples (several test groups and a control group) will be digested with Trypsin, and peptides will be enriched using an appropriate method, followed by chemical labeling by either iTRAQ or TMT labeling method if a quantitative analysis is desired. Enriched (with or without labeling) peptideswill be cleaned using SCX HPLC and a bRPLC fraction to further generate the 2nd dimansion of separation which has been proved to significantly increase the coverage and depth of the Mass Spectrometry Analysis.

Data analysis, including data validation, visualization and quantification, are performed with commercial softwares and Complete Omics’ unique R packages and scripts. Report will be sent to you in Excel format as well as a summary in PDF format. We will also provide you any details you need for your papers’ MATERIALS AND METHODS section. We will make sure you understand your result and help you with your paper writing with free follow-up services.

Sample types we accept:

1, 2D gel spots, SDS-PAGE bands

2, Cell Lysates and Tissue Lysates

3, Biofluids, such as plasma*, serum*, saliva, tear, etc.

*We provide High Abundance Protein Depletion Service to significantly (100-500 folds) increase the depth of your biofluid proteomics analysis by removing top abundant proteins from your samples. Read more for details​.

4, FFPE slide/ FFPE extract

5, Customized sample types (please contact us to discuss)

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