Introduction:

This is one of the most popular services in Complete Omics Proteomics Services, and our service allows you to take full advantage of our most advanced Orbitrap Mass Spectrometers to identify your complete catalogue of the proteins present in your complex biological samples, such as cell lysates, tissue lysates, serum, plasma and other complicated samples. Proteins in your samples are digested with a Complete Omics unique combination of proteases, Trypsin and LysC. 2D fractionation is performed to further fractionate your samples into appropriate number of fractiontions according to your samples’ complexicity. Peptides in total sample or each fraction are analyzed by world’s most advanced Orbitrap LC-MS/MS. Identified peptides from your samples/fractions are matched to protein sequence database.

Data analysis, including data validation, visualization and quantification, are performed with commercial softwares and Complete Omics’ unique R packages and scripts. Report will be sent to you in Excel format as well as a summary in PDF format. We will also provide you any details you need for your papers’ MATERIALS AND METHODS section. We will make sure you understand your result and help you with your paper writing with free follow-up services.

Sample types we accept:

1, 2D gel spots, SDS-PAGE bands

2, Cell Lysates and Tissue Lysates

3, Biofluids, such as plasma*, serum*, saliva, tear, etc.

*We provide High Abundance Protein Depletion Service to significantly (100-500 folds) increase the depth of your biofluid proteomics analysis by removing top abundant proteins from your samples. Read more for details​.

4, FFPE slide/ FFPE extract

5, Customized sample types (please contact us to discuss)

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